Influence of artificial saliva in biofilm formation of Candida albicans in vitro
| Autor: | Adeline Lacerda Jorjão, Juliana Campos Junqueira, Michelle Peneluppi Silva, José Chibebe Junior, Luciane Dias de Oliveira, Ana Karina da Silva Machado, Antonio Olavo Cardoso Jorge |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Saliva
food.ingredient Serial dilution Acrylic Resins Colony Count Microbial Microbiology chemistry.chemical_compound fluids and secretions food Candida albicans Humans Agar General Materials Science Chromatography biology Lactoferrin Biofilm Lactoperoxidase Saliva Artificial biology.organism_classification lcsh:RK1-715 stomatognathic diseases Distilled water chemistry Biofilms lcsh:Dentistry Artificial biology.protein Lysozyme |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Oral Research v.26 n.1 2012 Brazilian Oral Research Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO Brazilian Oral Research, Vol 26, Iss 1, Pp 24-28 (2012) Brazilian Oral Research, Volume: 26, Issue: 1, Pages: 24-28, Published: FEB 2012 |
| ISSN: | 1806-8324 |
| Popis: | Made available in DSpace on 2013-08-12T19:14:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T18:34:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:38Z No. of bitstreams: 1 S1806-83242012000100005.pdf: 1422001 bytes, checksum: d97d474bfd51d1785137db3a84b04680 (MD5) Made available in DSpace on 2014-05-20T14:04:38Z (GMT). No. of bitstreams: 1 S1806-83242012000100005.pdf: 1422001 bytes, checksum: d97d474bfd51d1785137db3a84b04680 (MD5) Previous issue date: 2012-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 degrees C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 10(6) cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 degrees C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 degrees C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p |
| Databáze: | OpenAIRE |
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