Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats
| Autor: | Tânia A. T. Gomes, Fabiana C. Moreira, JL Menchaca-Diaz, Ivan Hong Jun Koh, Ama Liberatore |
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| Rok vydání: | 2011 |
| Předmět: |
Physiology
Immunology Biophysics Spleen Ileum Biology Biochemistry Microbiology Microcirculation Enteropathogenic Escherichia coli parasitic diseases medicine Animals Humans Mesenteric lymph nodes Mesentery Rats Wistar General Pharmacology Toxicology and Pharmaceutics Child Escherichia coli Infections Colony-forming unit General Neuroscience Cell Biology General Medicine Rats Intestines medicine.anatomical_structure Liver Bacterial Translocation Duodenum Female Lymph Nodes Intravital microscopy |
| Zdroj: | Brazilian Journal of Medical and Biological Research. 44:1018-1024 |
| ISSN: | 0100-879X |
| Popis: | Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in mesenteric and abdominal organs by intravital microscopy and laser Doppler flow, respectively. tEPEC (N = 11) and aEPEC (N = 11) were recovered from MLN (100%), spleen (36.4 and 45.5%), and liver (45.5 and 72.7%) of the animals, respectively. Recovery of the positive control E. coli R-6 (N = 6) was 100% for all compartments. Bacteria were not recovered from extraintestinal sites of controls inoculated with non-pathogenic E. coli strains HB101 (N = 6) and HS (N = 10), or saline. Mesenteric microcirculation injuries were detected with both EPEC strains, but only aEPEC was similar to E. coli R-6 with regard to systemic tissue hypoperfusion. In conclusion, overgrowth of certain aEPEC strains may lead to BT and impairment of the microcirculation in systemic organs. |
| Databáze: | OpenAIRE |
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