Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants
| Autor: | Victor Won Tat Liu, Vimla Band, Aharon Solomon, Cesar Ortega-Cava, Yousaf Mian, Hamid Band, Channabasavaiah B. Gurumurthy, Mayumi Naramura |
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| Jazyk: | angličtina |
| Předmět: |
lin-12/Notch
Transgene Mutant Notch signaling pathway Cell fate determination 03 medical and health sciences let-7 microRNA Molecular Biology Caenorhabditis elegans 030304 developmental biology lin-41 0303 health sciences biology 030302 biochemistry & molecular biology fungi Cell Biology biology.organism_classification Molecular biology Phenotype Notch proteins Cell fate Heterochronic genes microRNA molting Seam cells Developmental timing Developmental Biology |
| Zdroj: | Developmental Biology. (2):191-199 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2007.12.046 |
| Popis: | The lin-12/Notch signaling pathway is conserved from worms to humans and is a master regulator of metazoan development. Here, we demonstrate that lin-12/Notch gain-of-function (gf) animals display precocious alae at the L4 larval stage with a significant increase in let-7 expression levels. Furthermore, lin-12(gf) animals display a precocious and higher level of let-7 gfp transgene expression in seam cells at L3 stage. Interestingly, lin-12(gf) mutant rescued the lethal phenotype of let-7 mutants similar to other known heterochronic mutants. We propose that lin-12/Notch signaling pathway functions in late developmental timing, upstream of or in parallel to the let-7 heterochronic pathway. Importantly, the human microRNA let-7a was also upregulated in various human cell lines in response to Notch1 activation, suggesting an evolutionarily conserved cross-talk between let-7 and the canonical lin-12/Notch signaling pathway. |
| Databáze: | OpenAIRE |
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