Conformational changes in the cytochrome b6f complex induced by inhibitor binding
| Autor: | Cécile Breyton |
|---|---|
| Přispěvatelé: | Thomas, Frank, Max Planck Institute of Biophysics [Frankfurt am Main] (MPIBP), Max-Planck-Gesellschaft |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
MESH: Chlamydomonas reinhardtii
Stereochemistry Protein Conformation [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Transmembrane Region Polyenes Biochemistry chemistry.chemical_compound Protein structure MESH: Protein Conformation MESH: Polyenes MESH: Cytochrome b Group Animals MESH: Animals Enzyme Inhibitors Molecular Biology Heme biology [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Cytochrome b6f complex Stigmatellin Cytochrome c MESH: Cytochrome b6f Complex Cell Biology Cytochrome b Group Crystallography Cytochrome b6f Complex chemistry MESH: Enzyme Inhibitors biology.protein Rieske protein Chlamydomonas reinhardtii |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, 2000, 275 (18), pp.13195-201 |
| ISSN: | 0021-9258 1083-351X |
| Popis: | International audience; Binding of stigmatellin, an inhibitor of the Q(o) site of the bc-type complexes, has been shown to induce large conformational changes of the Rieske protein in the respiratory bc(1) complex (Kim, H., Xia, D., Yu, C. A., Xia, J. Z., Kachurin, A. M., Zhang, L., Yu, L., and Deisenhofer, J. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 8026-8033; Iwata, S., Lee, J. W., Okada, K., Lee, J. K., Iwata, M., Rasmussen, B., Link, T. A., Ramaswamy, S., and Jap, B. K. (1998) Science 281, 64-71; Zhang, Z., Huang, L., Shulmeister, V. M., Chi, Y. I., Kim, K. K., Hung, L. W., Crofts, A. R., Berry, E. A., and Kim, S. H. (1998) Nature 392, 677-684). Such a movement seems necessary to shuttle electrons from the membrane-soluble quinol to the extramembrane heme of cytochrome c(1). To see whether similar changes occur in the related photosynthetic b(6)f complex, we have studied the effect of the binding of stigmatellin to the eukaryotic b(6)f complex by electron crystallography. Comparison of projection maps of thin three-dimensional crystals prepared with or without stigmatellin, and either negatively stained or embedded in glucose, reveals a similar type of movement to that observed in the bc(1) complex and suggests also the occurrence of conformational changes in the transmembrane region. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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