Transcriptomic profiling of a chicken lung epithelial cell line (CLEC213) reveals a mitochondrial respiratory chain activity boost during influenza virus infection
| Autor: | Bernard Delmas, Pascale Quéré, Ronan Le Goffic, Philippe Roingeard, Léa Meyer, Olivier Leymarie, Sonia Georgeault, Christophe Chevalier, Marco Moroldo, Bruno Da Costa, Evelyne Esnault |
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| Přispěvatelé: | Unité de recherche Virologie et Immunologie Moléculaires (VIM), Institut National de la Recherche Agronomique (INRA), Université Paris-Saclay, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Centres de Ressources Biologiques pour les Animaux Domestiques (CRB-Anim ), Université Francois Rabelais [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), GIS IBiSA, U 966, Institut National de la Santé et de la Recherche Médicale (INSERM), Conseil Regional d’Ile-de-France through DIM Malinf ('Domaine d'Intérêt Majeur Maladies Infectieuses, parasitaires et nosocomiales émergentes', Grant#: dim100157), Animal Health Division of INRA, 'DIM Malinf', Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Metabolic Processes Pulmonology Microarrays Virologie Respiratory chain lcsh:Medicine Gene Expression Mitochondrion medicine.disease_cause Pathology and Laboratory Medicine Virus Replication Biochemistry Oxidative Phosphorylation Poultry Epithelium Transcriptome 0302 clinical medicine Animal Cells Influenza A virus virus influenza aviaire Medicine and Health Sciences Gamefowl Enzyme Inhibitors lcsh:Science Immune Response Lung Energy-Producing Organelles Multidisciplinary Cell biology Mitochondria lignée cellulaire Mitochondrial respiratory chain Bioassays and Physiological Analysis [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Vertebrates Cellular Structures and Organelles Cellular Types Anatomy acide nucléique Autre (Sciences du Vivant) Research Article [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Immunology virus Biology Bioenergetics Research and Analysis Methods Virus Cell Line Birds Electron Transport 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Virology medicine Genetics Animals Inflammation transcriptomique Gene Expression Profiling lcsh:R Organisms Biology and Life Sciences Epithelial Cells Cell Biology Influenza A virus subtype H5N1 nucleic acid volaille 030104 developmental biology Metabolism Biological Tissue Viral replication Fowl Influenza in Birds Amniotes Respiratory Infections lcsh:Q Oligomycins infectiologie Chickens 030215 immunology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2017, 12 (4), pp.1-18. ⟨10.1371/journal.pone.0176355⟩ Plos One 4 (12), 1-18. (2017) PLoS ONE, Public Library of Science, 2017, 12 (4), 18 p. ⟨10.1371/journal.pone.0176355⟩ PLoS ONE, Vol 12, Iss 4, p e0176355 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Current Adress: Olivier Leymarie, Institut Cochin, INSERM U 1016, CNRS UMR 8104, Université Paris Descartes, Paris, FranceWe thank Jérôme Lecardonnel for technical assistance in microarrays hybridization.Dr Mariette Ducatez and Dr Earl Brown are gratefully acknowledged for the gift of the H7N1 reverse genetic system and the pgHH21-vNA-Luc plasmid respectively.; International audience; Avian Influenza virus (AIV) is a major concern for the global poultry industry. Since 2012, several countries have reported AIV outbreaks among domestic poultry. These outbreaks had tremendous impact on poultry production and socio-economic repercussion on farmers. In addition, the constant emergence of highly pathogenic AIV also poses a significant risk to human health. In this study, we used a chicken lung epithelial cell line (CLEC213) to gain a better understanding of the molecular consequences of low pathogenic AIV infection in their natural host. Using a transcriptome profiling approach based on microarrays, we identified a cluster of mitochondrial genes highly induced during the infection. Interestingly, most of the regulated genes are encoded by the mitochondrial genome and are involved in the oxidative phosphorylation metabolic pathway. The biological consequences of this transcriptomic induction result in a 2.5- to 4-fold increase of the ATP concentration within the infected cells. PB1-F2, a viral protein that targets the mitochondria was not found associated to the boost of activity of the respiratory chain. We next explored the possibility that ATP may act as a host-derived danger signal (through production of extracellular ATP) or as a boost to increase AIV replication. We observed that, despite the activation of the P2X7 purinergic receptor pathway, a 1mM ATP addition in the cell culture medium had no effect on the virus replication in our epithelial cell model. Finally, we found that oligomycin, a drug that inhibits the oxidative phosphorylation process, drastically reduced the AIV replication in CLEC213 cells, without apparent cellular toxicity. Collectively, our results suggest that AIV is able to boost the metabolic capacities of its avian host in order to provide the important energy needs required to produce progeny virus. |
| Databáze: | OpenAIRE |
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