Comparative proteomics of glycosomes from bloodstream form and procyclic culture formTrypanosoma brucei brucei
| Autor: | Frank Voncken, Claudia Colasante, Margrit Ellis, Thomas Ruppert |
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| Rok vydání: | 2006 |
| Předmět: |
Proteomics
biology Trypanosoma brucei brucei Cell Culture Techniques Protozoan Proteins Kinetoplastida Peroxisome Trypanosoma brucei biology.organism_classification Microbodies Biochemistry Glycosome Mass Spectrometry Host-Parasite Interactions Cell biology parasitic diseases Organelle Proteome Animals Protozoa Glycolysis Molecular Biology |
| Zdroj: | PROTEOMICS. 6:3275-3293 |
| ISSN: | 1615-9861 1615-9853 |
| DOI: | 10.1002/pmic.200500668 |
| Popis: | Peroxisomes are present in nearly every eukaryotic cell and compartmentalize a wide range of important metabolic processes. Glycosomes of Kinetoplastid parasites are peroxisome-like organelles, characterized by the presence of the glycolytic pathway. The two replicating stages of Trypanosoma brucei brucei, the mammalian bloodstream form (BSF) and the insect (procyclic) form (PCF), undergo considerable adaptations in metabolism when switching between the two different hosts. These adaptations involve also substantial changes in the proteome of the glycosome. Comparative (non-quantitative) analysis of BSF and PCF glycosomes by nano LC-ESI-Q-TOF-MS resulted in the validation of known functional aspects of glycosomes and the identification of novel glycosomal constituents. |
| Databáze: | OpenAIRE |
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