Adenomatous polyposis coli-binding protein end-binding 1 promotes hepatocellular carcinoma growth and metastasis

Autor: Akinobu Taketomi, Moto Fukai, Hideki Yokoo, Toshiya Kamiyama, Kanako C. Hatanaka, Takeshi Aiyama, Yutaka Hatanaka, Tatsuya Orimo, Takanori Ohata
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Colorectal cancer
Cellular differentiation
Gene Expression
Kaplan-Meier Estimate
medicine.disease_cause
Metastasis
Gene Knockout Techniques
0302 clinical medicine
Recurrence
Breast Tumors
Basic Cancer Research
Medicine and Health Sciences
RNA
Neoplasm
Neoplasm Metastasis
RNA
Small Interfering
Gene knockdown
Mice
Inbred BALB C
Multidisciplinary
Portal Vein
Liver Diseases
Liver Neoplasms
Cell Differentiation
Middle Aged
Prognosis
Recombinant Proteins
Neoplasm Proteins
Gene Expression Regulation
Neoplastic
Survival Rate
Oncology
Cell Processes
030220 oncology & carcinogenesis
Medicine
Heterografts
Female
RNA Interference
Microtubule-Associated Proteins
Research Article
Adult
Carcinoma
Hepatocellular
Genes
APC
Hepatitis
Viral
Human
Adenomatous polyposis coli
Science
Mice
Nude
macromolecular substances
Gastroenterology and Hepatology
Biology
03 medical and health sciences
Cell Line
Tumor
Gastrointestinal Tumors
Breast Cancer
Carcinoma
medicine
Genetics
Animals
Humans
Neoplasm Invasiveness
RNA
Messenger
Aged
Cell Proliferation
Colorectal Cancer
Microarray analysis techniques
fungi
Cancers and Neoplasms
Biology and Life Sciences
Hepatocellular Carcinoma
Cell Biology
medicine.disease
digestive system diseases
Gastric Cancer
030104 developmental biology
Tissue Array Analysis
biology.protein
Cancer research
Carcinogenesis
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 9, p e0239462 (2020)
ISSN: 1932-6203
Popis: This study was performed to determine the clinical significance of adenomatous polyposis coli (APC)-binding protein end-binding 1 (EB1) in hepatocellular carcinoma (HCC) and to characterize its biochemical role in comparison with previous reports. We performed immunohistochemical staining to detect EB1 expression in tissues from 235 patients with HCC and investigated its correlations with clinicopathological features and prognosis. We also investigated the roles of EB1 in cell proliferation, migration, and tumorigenesis in vitro and in vivo by siRNA- and CRISPR/Cas9-mediated modulation of EB1 expression in human HCC cell lines. The results showed that EB1 expression was significantly correlated with several important factors associated with tumor malignancy, including histological differentiation, portal vein invasion status, and intrahepatic metastasis. Patients with high EB1 expression in HCC tissue had poorer overall survival and higher recurrence rates than patients with low EB1 expression. EB1 knockdown and knockout in HCC cells reduced cell proliferation, migration, and invasion in vitro and inhibited tumor growth in vivo. Further, genes encoding Dlk1, HAMP, and SLCO1B3 that were differentially expressed in association with EB1 were identified using RNA microarray analysis. In conclusion, elevated expression of EB1 promotes tumor growth and metastasis of HCC. EB1 may serve as a new biomarker for HCC, and genes coexpressed with EB1 may represent potential targets for therapy.
Databáze: OpenAIRE
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