Development of dynamic image analysis methods to measure vascularisation and syncytial nuclear aggregates in human placenta

Autor:	Alan Kerby, Nicole Graham, Raianne Wallworth, Gauri Batra, Alexander Heazell
Rok vydání:	2021
Předmět:	Reproductive Medicine Neovascularization Pathologic Pregnancy Placenta Image Processing Computer-Assisted Obstetrics and Gynecology Endothelial Cells Humans Female Software Developmental Biology
Zdroj:	Placenta. 120
ISSN:	1532-3102
Popis:	Histological examination of the placenta significantly contributes to diagnosis in adverse birth outcomes. One challenge in image analysis is variation in staining intensity caused by batch variation. We investigated if dynamic threshold image analysis methods may increase accuracy. Placenta samples were stained for endothelial cells and syncytial nuclear aggregates and analysed in Qupath software. Dynamically setting the threshold resulted in data more similar to manual method data. The method is simple and effective at modelling the dynamic interpretation of variation in staining intensity achieved by manual methods. We anticipate dynamic methods could be used to enhance placental diagnosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01fe604254ab4de0a3bebad91240f04a https://pubmed.ncbi.nlm.nih.gov/35220183 Zobrazit plný text záznamu Full Text from ScienceDirect