Production of uracil from methane by a newly isolated Methylomonas sp. SW1
| Autor: | Han Bin Oh, Byung-Keun Oh, Jinwon Lee, Yuhyun Kwon, Sukhyeong Cho, Seokhun Yun, Soohyun Park, Insu Song, Sangwoo Kim, Wangjun Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sewage chemistry.chemical_element Bioengineering Methylomonas 010501 environmental sciences Methylomonas sp Raw material 01 natural sciences Applied Microbiology and Biotechnology Methane 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Republic of Korea Uracil 0105 earth and related environmental sciences Bacteriological Techniques biology business.industry General Medicine biology.organism_classification RNA Bacterial 030104 developmental biology chemistry Biochemistry Environmental chemistry business Carbon Bacteria Biotechnology |
| Zdroj: | Journal of biotechnology. 240 |
| ISSN: | 1873-4863 |
| Popis: | Methane is an abundant, inexpensive one-carbon feedstock and one of the most powerful greenhouse gases. Because it does not compete with food demand, it is considered a promising carbon feedstock for the production of valuable products using methanotrophic bacteria. Here, we isolated a novel methanotrophic bacterium, Methylomonas sp. SW1, from a sewage sample obtained from Wonju City Water Supply Drainage Center, Republic of Korea. The conditions for uracil production by Methylomonas sp. SW1, such as Cu2+ concentration and temperature were investigated and optimized. As a result, Methylomonas sp. SW1 produced uracil from methane as a sole carbon source with a titer of 2.1mg/L in 84h without genetic engineering under the optimized condition. The results in this study demonstrate the feasibility of using Methylomonas sp. SW1 for the production of uracil from methane. This is the first report of uracil production from gas feedstock by methanotrophic bacteria. |
| Databáze: | OpenAIRE |
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