Maternal platelets pass interstices of trophoblast columns and are not activated by HLA-G in early human pregnancy
| Autor: | Shrey Kohli, Heinz Hutter, S. Wernitznig, Beatrice A. Brugger, Berend Isermann, Sabine Maninger, Nadja Kupper, Jacqueline Guettler, Désirée Forstner, Herbert Juch, Elisabeth Pritz, Gerhard Cvirn, Gottfried Dohr, Olivia Nonn, Martin Gauster |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Blood Platelets Placenta Immunology Primary Cell Culture Cell Line Andrology 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission Pregnancy HLA-G medicine Immunology and Allergy Humans Platelet Placental Circulation Maternal-Fetal Exchange reproductive and urinary physiology HLA-G Antigens 030219 obstetrics & reproductive medicine Chemistry Obstetrics and Gynecology Trophoblast Cell Differentiation Intervillous space Adhesion medicine.disease Coculture Techniques Recombinant Proteins Trophoblasts Pregnancy Trimester First 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Immunohistochemistry Female |
| Zdroj: | Journal of reproductive immunology. 144 |
| ISSN: | 1872-7603 |
| Popis: | In early human gestation, maternal arterial blood flow into the intervillous space of the developing placenta is obstructed by invaded trophoblasts, which form cellular plugs in uterine spiral arteries. These trophoblast plugs have recently been described to be loosely cohesive with clear capillary-sized channels into the intervillous space by 7 weeks of gestation. Here, we analysed localisation of maternal platelets at the maternal-foetal interface of human first trimester pregnancy, and tested the hypothesis whether HLA-G, which is primarily expressed by extravillous trophoblasts, affects aggregation and adhesion of isolated platelets. Immunohistochemistry of first trimester placental sections localised maternal platelets in vessel-like channels and adjacent intercellular gaps of extravillous trophoblasts in distal parts of columns. Furthermore, this localisation was confirmed by transmission electron microscopy. Neither co-incubation of HLA-G overexpressing JAR cells with isolated platelets, nor incubation with cell-derived soluble HLA-G or recombinant HLA-G affected platelet adhesion and aggregation. Our study suggests that maternal platelets flow through vessel-like channels of distal trophoblast columns and spread into adjacent lateral intercellular gaps, where platelet-derived factors could contribute to trophoblast differentiation into the invasive phenotype. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect