A Full Set of In Vitro Assays in Chitosan/Tween 80 Microspheres Loaded with Magnetite Nanoparticles
| Autor: | Celia N Sánchez-Domínguez, Karla V Sosa-Cruz, Víctor Gómez-Flores, Jorge A Roacho-Pérez, Hugo L. Gallardo-Blanco, Christian Chapa-Gonzalez, Perla E. García-Casillas, María R Velazco-Campos, Kassandra O Rodríguez-Aguillón, Ana María Rivas-Estilla, Luz Rojas-Patlan, Margarita Sanchez-Dominguez |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Thermogravimetric analysis
Tween 80 Polymers and Plastics synthesis Cell morphology Article Chitosan lcsh:QD241-441 chemistry.chemical_compound Dynamic light scattering lcsh:Organic chemistry medicine Bovine serum albumin Cell damage polymers chemistry.chemical_classification Chromatography biology genotoxicity General Chemistry Polymer medicine.disease magnetite nanoparticles chemistry Nanotoxicology biology.protein nanotoxicology chitosan hemotoxicity |
| Zdroj: | Polymers Volume 13 Issue 3 Polymers, Vol 13, Iss 400, p 400 (2021) |
| ISSN: | 2073-4360 |
| Popis: | Microspheres have been proposed for different medical applications, such as the delivery of therapeutic proteins. The first step, before evaluating the functionality of a protein delivery system, is to evaluate their biological safety. In this work, we developed chitosan/Tween 80 microspheres loaded with magnetite nanoparticles and evaluated cell damage. The formation and physical–chemical properties of the microspheres were determined by FT-IR, Raman, thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDS), dynamic light scattering (DLS), and SEM. Cell damage was evaluated by a full set of in vitro assays using a non-cancerous cell line, human erythrocytes, and human lymphocytes. At the same time, to know if these microspheres can load proteins over their surface, bovine serum albumin (BSA) immobilization was measured. Results showed 7 nm magnetite nanoparticles loaded into chitosan/Tween 80 microspheres with average sizes of 1.431 µm. At concentrations from 1 to 100 µg/mL, there was no evidence of changes in mitochondrial metabolism, cell morphology, membrane rupture, cell cycle, nor sister chromatid exchange formation. For each microgram of microspheres 1.8 µg of BSA was immobilized. The result provides the fundamental understanding of the in vitro biological behavior, and safety, of developed microspheres. Additionally, this set of assays can be helpful for researchers to evaluate different nano and microparticles. |
| Databáze: | OpenAIRE |
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