Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes

Autor: Kevin F. Sullivan, Eng M. Tan, Michael B. Prystowsky, P. M. Shipman, P. G. Comber, Daniel E. Sabath, A. H. Fischer
Rok vydání: 1988
Předmět:
Zdroj: Journal of Cellular Biochemistry. 38:189-198
ISSN: 1097-4644
0730-2312
DOI: 10.1002/jcb.240380306
Popis: Expression of cyclin, a non-histone nuclear protein, during recombinant interleukin 2 (rIL2)-driven cell-cycle progression of cloned T lymphocytes has been assessed. We found that expression of cyclin protein, as detected by immunofluorescence, is tightly associated with proliferation, and not merely S-phase, of L2 cells stimulated with rIL2. Cyclin immunofluorescence was detected in all cell-cycle phases (G1/S/G2/M, as detected by flow cytometry) of proliferating L2 cells. Accumulation of cyclin mRNA levels was induced as early as 1 h after stimulation, was maximal at 25-49 h, and remained elevated throughout stimulation, as detected by Northern blot analysis. A cDNA-encoding murine cyclin was cloned from a cDNA library prepared from IL2-stimulated cloned T cells. The sequence of the 5' end of the murine cyclin cDNA was determined and found to be 88% and 82% similar to the sequences of cDNA clones encoding rat and human cyclin, respectively. The present studies demonstrate that cyclin protein and mRNA accumulation are highly regulated during IL2-induced proliferation of a cloned T cell. These data provide a framework for addressing the molecular mechanisms regulating cyclin gene expression during cellular proliferation.
Databáze: OpenAIRE
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