Sample Preparation for Mass Cytometry Analysis
Autor: | Michelle Craig Barton, Ryan L. McCarthy, Aundrietta D. Duncan |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Protocol (science) General Immunology and Microbiology General Chemical Engineering General Neuroscience Tumor cells Cell Separation Biology Bioinformatics computer.software_genre Antibodies General Biochemistry Genetics and Molecular Biology Specimen Handling Mice Cellular Biology 03 medical and health sciences 030104 developmental biology Metals Heavy Tumor Cells Cultured Animals Sample preparation Mass cytometry Data mining computer |
Zdroj: | Journal of Visualized Experiments. |
ISSN: | 1940-087X |
Popis: | Mass cytometry utilizes antibodies conjugated with heavy metal labels, an approach that has greatly increased the number of parameters and opportunities for deep analysis well beyond what is possible with conventional fluorescence-based flow cytometry. As with any new technology, there are critical steps that help ensure the reliable generation of high-quality data. Presented here is an optimized protocol that incorporates multiple techniques for the processing of cell samples for mass cytometry analysis. The methods described here will help the user avoid common pitfalls and achieve consistent results by minimizing variability, which can lead to inaccurate data. To inform experimental design, the rationale behind optional or alternative steps in the protocol and their efficacy in uncovering new findings in the biology of the system being investigated is covered. Lastly, representative data is presented to illustrate expected results from the techniques presented here. |
Databáze: | OpenAIRE |
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