Identification and relative abundances of mRNA for a gene encoding the vWD domain and three Kazal-type domains in the ovary of giant freshwater prawns, Macrobrachium rosenbergii
| Autor: | Aslah Mohamad, Safiah Jasmani, Ariffin Hidir, Li Lian Wong, Mohd Pauzi Mardhiyyah, Mhd Ikhwanuddin, Zakaria Muhammad Faiz, Amirdin Nurul-Hidayah |
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| Rok vydání: | 2018 |
| Předmět: |
Sex Differentiation
Hepatopancreas Ovary Fresh Water Biology 03 medical and health sciences 0302 clinical medicine Endocrinology Food Animals Protein Domains Complementary DNA von Willebrand Factor medicine Animals Amino Acid Sequence RNA Messenger Sexual Maturation Cloning Molecular Gene Messenger RNA 030219 obstetrics & reproductive medicine Base Sequence Macrobrachium rosenbergii 0402 animal and dairy science Gene Expression Regulation Developmental 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040201 dairy & animal science Molecular biology Kazal Motifs Open reading frame medicine.anatomical_structure Real-time polymerase chain reaction Animal Science and Zoology Female Palaemonidae |
| Zdroj: | Animal reproduction science. 209 |
| ISSN: | 1873-2232 |
| Popis: | Understanding Macrobrachium rosenbergii ovarian maturation control at the genome level is an important aspect for increasing larvae production. In this study, an ovarian maturation related gene, M. rosenbergii vWD domain and three Kazal-type domains of a gene (MrvWD-Kazal) have been studied. The MrvWD-Kazal gene was isolated using a rapid amplification of cDNA end (RACE) method and the relative abundances of MrvWD-Kazal mRNA in the ovary, hepatopancreas, stomach, intestine and gill were determined by using the quantitative PCR technique. The MrvWD-Kazal gene is composed of 2194 bp with an open reading frame (ORF) of 1998 bp encoding 665 amino acids and has great similarity to the M. nipponense vWD-Kazal gene (91%). The qPCR analyses indicated the relative abundance of MrvWD-Kazal mRNA transcript varied among different stages of ovarian function (P0.05), but there were no differences abundance in hepatopancreas, stomach, intestine and gill (P0.05). In the ovary, relative abundance of MrvWD-Kazal mRNA transcript gradually increased with ovarian maturation from Stages 1 (Spent; 1.00-fold), to 2 (Proliferative; 3.47-fold) to 3 (Premature; 6.18-fold) and decreased at Stage 4 (Mature; 1.31-fold). Differential relative abundances of MrvWD-Kazal mRNA transcript in the ovary indicate the MrvWD-Kazal protein may have an important function in ovarian maturation of M. rosenbergii. The results of this study also indicate the MrvWD-Kazal is not involved in regulation of the reproductive related function of the hepatopancreas, digestive system (stomach and intestine) and respiratory system (gill). |
| Databáze: | OpenAIRE |
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