Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor
| Autor: | In-Pil Jung, Ho-Sup Jung, Na-Reum Ha, Im-Joung La, Moon-Young Yoon |
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| Rok vydání: | 2016 |
| Předmět: |
Aptamer
02 engineering and technology 01 natural sciences Article law.invention Blood serum law medicine Multidisciplinary Chromatography biology Chemistry Graphene business.industry 010401 analytical chemistry Kanamycin biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology biology.organism_classification Food safety Molecular biology Fluorescence 0104 chemical sciences 0210 nano-technology business Biosensor Bacteria medicine.drug |
| Zdroj: | Scientific Reports SCIENTIFIC REPORTS(7) |
| ISSN: | 2045-2322 |
| Popis: | Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products is urgently needed for food safety. In this study, we identified kanamycin-binding aptamers via a new screening method, and truncated variants were analyzed for optimization of the minimal sequence required for target binding. We found various aptamers with high binding affinity from 34.7 to 669 nanomolar Kdapp values with good specificity against kanamycin. Furthermore, we developed a reduced graphene oxide (RGO)-based fluorescent aptasensor for kanamycin detection. In this system, kanamycin was detected at a concentration as low as 1 pM (582.6 fg/mL). In addition, this method could detect kanamycin accurately in kanamycin-spiked blood serum and milk samples. Consequently, this simple, rapid, and sensitive kanamycin detection system with newly structural and functional analysis aptamer exhibits outstanding detection compared to previous methods and provides a new possibility for point of care testing and food safety. |
| Databáze: | OpenAIRE |
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