Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins
| Autor: | Clara Cassinelli, René Verhoef, Jean Luc Duval, Anne Bensoussan, Marco Morra, Pascale Vigneron, Marie Danielle Nagel, Cyrill Bussy, Giovanna Cascardo, Ash Haeger, Elodie Velzenberger, J. Paul Knox, Henk A. Schols |
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| Přispěvatelé: | Guilbert, Chantal |
| Rok vydání: | 2007 |
| Předmět: |
rhamnogalacturonan
hairy ramified regions Materials science Biocompatibility growth Biomedical Engineering polysaccharides Chick Embryo In Vitro Techniques Polysaccharide Extracellular matrix Mice Cell Movement Bone cell Levensmiddelenchemie Animals Secretion extracellular-matrix Cell Shape VLAG Cell Proliferation chemistry.chemical_classification Food Chemistry Tibia Tumor Necrosis Factor-alpha Macrophages Cell Cycle Metals and Alloys Cell migration Enzymes Immobilized In vitro osteoblast adhesion chemistry Biochemistry Ceramics and Composites Surface modification synthetic peptides Pectins Polystyrenes activation surface modification biomaterials |
| Zdroj: | Journal of Biomedical Materials Research Part A, 86a(3), 597-606 HAL Journal of Biomedical Materials Research Part A 86a (2008) 3 |
| ISSN: | 1552-4965 1549-3296 |
| Popis: | Previous work has reported the results of a multidisciplinary effort producing a proof-of-concept on the use of pectic polysaccharides in the surface modification of medical devices. This study was designed to learn more about the capability of engineered rhamnogalacturonan-I (RG-I) fractions of apple pectin to control bone cell and macrophage behavior. Thermanox® or polystyrene Petri dishes were surface modified with two different modified hairy regions (MHRs) obtained by different enzymatic liquefaction processes of apples differing in relative amounts and lengths of their neutral side chains: (long-haired) MHR- and (short-haired) MHR-B. Bone explants from 14-day-old chick embryos were cultured for 14 days on both pectic substrata. MHR-B promoted cell migration and differentiation, MHR- did not. On MHR-, J774.2 macrophages grew well, their percentage in G1 phase was decreased and in S phase increased, and they did not secrete either proinflammatory-cytokines or nitrites. Contrasting results were gained from macrophages on MHR-B, except for nitrite secretion. Thus, we conclude that coatings from tailored pectins show different biological activities in vitro and are potential innovative candidates for improving the biocompatibility of medical devices in various applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008 |
| Databáze: | OpenAIRE |
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