NCK1-AS1 enhances glioma cell proliferation, radioresistance and chemoresistance via miR-22-3p/IGF1R ceRNA pathway
| Autor: | Bo Wang, Qiling Xu, Tenglong Jin, Kai Wang, Yanyang He, Bingzhou Cui, Yazhou Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Mice Nude RM1-950 Biology Receptor IGF Type 1 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation IGF1R Cell Line Tumor Glioma Radioresistance medicine Animals Humans miR-22-3p Adaptor Proteins Signal Transducing Cell Proliferation Insulin-like growth factor 1 receptor Oncogene Proteins Pharmacology Mice Inbred BALB C Brain Neoplasms Cell growth Competing endogenous RNA NCK1-AS1 General Medicine medicine.disease Xenograft Model Antitumor Assays Long non-coding RNA MicroRNAs 030104 developmental biology Drug Resistance Neoplasm Apoptosis 030220 oncology & carcinogenesis Cancer research RNA Long Noncoding Therapeutics. Pharmacology |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 129, Iss, Pp 110395-(2020) |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2020.110395 |
| Popis: | Glioma is the deadliest disease in human central nerve system. Abnormal expression of long noncoding RNA (lncRNA) expression has been demontrated to be implicated in various cancers. The oncogenic role of lncRNA NCK1-AS1 has been validated in cervical cancer, wheras its role in glioma remians obscure. Our research findings suggested that NCK1-AS1 was upregulated in glioma tissues and cells. NCK1-AS1 deficiency hindered cell proliferation and enhanced cell apoptosis. Additionally, the chemoresistance and radioresistance of glioma cells were impaired by NCK1-AS1 depletion. Moreover, miR-22-3p, a downstream gene of NCK1-AS1, could weaken glioma cell chemoresistance and radioresistance. Similarly, IGF1R was the downstream target gene of miR-22-3p. Further mechanism and function assays demonstrated that NCK1-AS1 promoted glioma cell growth, chemoresistance and radioresistance via sponging miR-22-3p to upregulate IGF1R. Finally, the tumor facilitator function of NCK1-AS1 was also verified by in vivo experiments. Taken together, NCK1-AS1 contributes to glioma cell proliferation, radioresistance and chemoresistance via miR-22-3p/IGF1R ceRNA pathway, which might provide a new insight for improving the radiotherapy and chemotherapy treatments of glioma. |
| Databáze: | OpenAIRE |
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