Screen-Printed Polyaniline-Based Electrodes for the Real-Time Monitoring of Loop-Mediated Isothermal Amplification Reactions
| Autor: | David Gosselin, Jean Berthier, Maxime Gougis, Didier Chaussy, Fabrice Navarro, Mélissa Baque, Mohamed Naceur Belgacem, Pascal Mailley, Anne-Gaelle Bourdat |
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| Přispěvatelé: | Laboratoire Génie des procédés papetiers (LGP2 ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Microfluidics
Loop-mediated isothermal amplification Analytical chemistry 02 engineering and technology 010402 general chemistry 01 natural sciences Reference electrode Analytical Chemistry [SPI.MAT]Engineering Sciences [physics]/Materials Silver chloride chemistry.chemical_compound Polyaniline Potentiometric sensor [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Electrodes ComputingMilieux_MISCELLANEOUS Aniline Compounds DNA Electrochemical Techniques Nucleic acid amplification technique Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology 0104 chemical sciences Electrochemical gas sensor chemistry 0210 nano-technology Nucleic Acid Amplification Techniques |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2017, 89 (19), pp.10124-10128. ⟨10.1021/acs.analchem.7b02394⟩ Analytical Chemistry, 2017, 89 (19), pp.10124-10128. ⟨10.1021/acs.analchem.7b02394⟩ |
| ISSN: | 0003-2700 1520-6882 |
| DOI: | 10.1021/acs.analchem.7b02394⟩ |
| Popis: | Nucleic acid amplification testing is a very powerful method to perform efficient and early diagnostics. However, the integration of a DNA amplification reaction with its associated detection in a low-cost, portable, and autonomous device remains challenging. Addressing this challenge, the use of screen-printed electrochemical sensor is reported. To achieve the detection of the DNA amplification reaction, a real-time monitoring of the hydronium ions concentration, a byproduct of this reaction, is performed. Such measurements are done by potentiometry using polyaniline (PAni)-based working electrodes and silver/silver chloride reference electrodes. The developed potentiometric sensor is shown to enable the real-time monitoring of a loop-mediated isothermal amplification (LAMP) reaction with an initial number of DNA strands as low as 10 copies. In addition, the performance of this PAni-based sensor is compared to fluorescence measurements, and it is shown that similar results are obtained for both methods. |
| Databáze: | OpenAIRE |
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