Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster
| Autor: | Hiroshi Nishimasu, Osamu Nureki, Naoki Matsumoto, Luc Bonnefond, Satoshi Fukuhara, Ryuichiro Ishitani |
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| Rok vydání: | 2012 |
| Předmět: |
Transposable element
endocrine system Molecular Sequence Data Biophysics Piwi-interacting RNA Sequence alignment Biology Protein Sorting Signals Crystallography X-Ray Biochemistry Chromatography Affinity Protein Structure Secondary Conserved sequence Structural Biology Endoribonucleases Genetics Escherichia coli Animals Drosophila Proteins Amino Acid Sequence Peptide sequence Conserved Sequence urogenital system Condensed Matter Physics biology.organism_classification Cell biology Drosophila melanogaster Crystallization Communications biological sciences health occupations bacteria Crystallization Sequence Alignment Drosophila Protein Biogenesis |
| Zdroj: | Acta crystallographica. Section F, Structural biology and crystallization communications. 68(Pt 11) |
| ISSN: | 1744-3091 |
| Popis: | PIWI-interacting RNAs (piRNAs) bind PIWI proteins and silence transposons to maintain the genomic integrity of germ cells. Zucchini (Zuc), a phospholipase D superfamily member, is conserved among animals and is implicated in piRNA biogenesis. However, the underlying mechanism by which Zuc participates in piRNA biogenesis remains elusive.Drosophila melanogasterZuc (DmZuc) was expressed inEscherichia coli, purified and crystallized. X-ray diffraction data were collected to 1.75 Å resolution. The crystal belonged to space groupP21, with unit-cell parametersa= 55.0,b= 71.2,c= 56.3 Å, β = 107.9°. |
| Databáze: | OpenAIRE |
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