Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype
| Autor: | Huiying Wang, Si Wu, Li Zhao, Miao Yu, Rui Ye, Pengbo Sun, Di Wang, Shuang Bai, Shuhua Xi, Cuihong Wang, Yong Yue |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty QH301-705.5 Disease Proteomics Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry chronic obstructive pulmonary disease 03 medical and health sciences 0302 clinical medicine proteomics medicine Molecular Biosciences KEGG Biology (General) Molecular Biology Original Research COPD Lung business.industry DHRS9 medicine.disease Phenotype respiratory tract diseases emphysematous phenotype Blot 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Wound healing business KRT17 |
| Zdroj: | Frontiers in Molecular Biosciences, Vol 8 (2021) Frontiers in Molecular Biosciences |
| DOI: | 10.3389/fmolb.2021.650604/full |
| Popis: | Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease. Emphysematous phenotype is the most common and critical phenotype, which is characterized by progressive lung destruction and poor prognosis. However, the underlying mechanism of this structural damage has not been completely elucidated. A total of 12 patients with COPD emphysematous phenotype (COPD-E) and nine patients with COPD non-emphysematous phenotype (COPD-NE) were enrolled to determine differences in differential abundant protein (DAP) expression between both groups. Quantitative tandem mass tag–based proteomics was performed on lung tissue samples of all patients. A total of 29 and 15 lung tissue samples from patients in COPD-E and COPD-NE groups, respectively, were used as the validation cohort to verify the proteomic analysis results using western blotting. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted for DAPs. A total of 4,343 proteins were identified, of which 25 were upregulated and 11 were downregulated in the COPD-E group. GO and KEGG analyses showed that wound repair and retinol metabolism–related pathways play an essential role in the molecular mechanism of COPD emphysematous phenotype. Three proteins, namely, KRT17, DHRS9, and FMO3, were selected for validation. While KRT17 and DHRS9 were highly expressed in the lung tissue samples of the COPD-E group, FMO3 expression was not significantly different between both groups. In conclusion, KRT17 and DHRS9 are highly expressed in the lung tissue of patients with COPD emphysematous phenotype. Therefore, these proteins might involve in wound healing and retinol metabolism in patients with emphysematous phenotype and can be used as phenotype-specific markers. |
| Databáze: | OpenAIRE |
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