Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE
| Autor: | Holli M. Drendel, Jill L. Reiter, Megan M. Schellinger, Gil Mor, Sujata Chakraborty, Men-Jean Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Telomerase Karyotype Biology Virus Cell Line 03 medical and health sciences chemistry.chemical_compound Cytogenetics 0302 clinical medicine medicine Humans Telomerase reverse transcriptase 030219 obstetrics & reproductive medicine Obstetrics and Gynecology Microsatellite instability medicine.disease Molecular biology Tumor antigen Trophoblasts 030104 developmental biology Reproductive Medicine chemistry Cell culture Microsatellite Instability DNA Developmental Biology |
| Zdroj: | Placenta. 52 |
| ISSN: | 1532-3102 |
| Popis: | Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells. |
| Databáze: | OpenAIRE |
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