Transcriptomic Analyses of Root Restriction Effects on Phytohormone Content and Signal Transduction during Grape Berry Development and Ripening

Autor: Ling Jiang, Chongde Sun, Xian Li, Jinping Cao, Shiping Wang, Feng Leng
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Biology
01 natural sciences
Plant Roots
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
Plant Growth Regulators
Auxin
Gene Expression Regulation
Plant
Brassinosteroid
Vitis
grape development
RNA-Seq
Physical and Theoretical Chemistry
skin and connective tissue diseases
Molecular Biology
Abscisic acid
lcsh:QH301-705.5
Spectroscopy
chemistry.chemical_classification
Jasmonic acid
Gene Expression Profiling
Organic Chemistry
fungi
food and beverages
Gene Expression Regulation
Developmental
Ripening
General Medicine
Computer Science Applications
phytohormones
030104 developmental biology
chemistry
Biochemistry
lcsh:Biology (General)
lcsh:QD1-999
Fruit
Cytokinin
root restriction
Gibberellin
sense organs
Salicylic acid
010606 plant biology & botany
Signal Transduction
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 8, p 2300 (2018)
Volume 19
Issue 8
ISSN: 1422-0067
Popis: Phytohormones strongly influence growth, development and nutritional quality of agricultural products by modulating molecular and biochemical changes. The purpose of the present study was to investigate the influence of root restriction (RR) treatment on the dynamic changes of main phytohormones during the berry development and ripening of &ldquo
Summer Black&rdquo
early ripening seedless grape (Vitis vinifera ×
V. labrusca), and to analyze the changes in the biosynthesis and signal transduction pathways of phytohormones by transcriptomics. Enzyme-linked immunosorbent assay (ELISA) and Ultra Performance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS) were used to quantify the phytohormone levels, and RNA-Seq was used to analyze the transcript abundance. The results showed that 23 transcripts involved in the phytohormone biosynthesis and 34 transcripts involved in the signal transduction pathways were significantly changed by RR treatment. RR also increased abscisic acid, brassinosteroid, ethylene, jasmonic acid and salicylic acid levels, while decreasing auxin, cytokinin, and gibberellin contents. The results of the present study suggest that RR treatment can accelerate the grape ripening process, and specific candidate genes were identified for further functional analysis.
Databáze: OpenAIRE
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