Transcriptomic Analyses of Root Restriction Effects on Phytohormone Content and Signal Transduction during Grape Berry Development and Ripening
Autor: | Ling Jiang, Chongde Sun, Xian Li, Jinping Cao, Shiping Wang, Feng Leng |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Biology 01 natural sciences Plant Roots Catalysis Article Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Auxin Gene Expression Regulation Plant Brassinosteroid Vitis grape development RNA-Seq Physical and Theoretical Chemistry skin and connective tissue diseases Molecular Biology Abscisic acid lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification Jasmonic acid Gene Expression Profiling Organic Chemistry fungi food and beverages Gene Expression Regulation Developmental Ripening General Medicine Computer Science Applications phytohormones 030104 developmental biology chemistry Biochemistry lcsh:Biology (General) lcsh:QD1-999 Fruit Cytokinin root restriction Gibberellin sense organs Salicylic acid 010606 plant biology & botany Signal Transduction |
Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 19, Iss 8, p 2300 (2018) Volume 19 Issue 8 |
ISSN: | 1422-0067 |
Popis: | Phytohormones strongly influence growth, development and nutritional quality of agricultural products by modulating molecular and biochemical changes. The purpose of the present study was to investigate the influence of root restriction (RR) treatment on the dynamic changes of main phytohormones during the berry development and ripening of &ldquo Summer Black&rdquo early ripening seedless grape (Vitis vinifera × V. labrusca), and to analyze the changes in the biosynthesis and signal transduction pathways of phytohormones by transcriptomics. Enzyme-linked immunosorbent assay (ELISA) and Ultra Performance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS) were used to quantify the phytohormone levels, and RNA-Seq was used to analyze the transcript abundance. The results showed that 23 transcripts involved in the phytohormone biosynthesis and 34 transcripts involved in the signal transduction pathways were significantly changed by RR treatment. RR also increased abscisic acid, brassinosteroid, ethylene, jasmonic acid and salicylic acid levels, while decreasing auxin, cytokinin, and gibberellin contents. The results of the present study suggest that RR treatment can accelerate the grape ripening process, and specific candidate genes were identified for further functional analysis. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |