In Vitro and In Vivo Electrochemical Measurement of Reactive Oxygen Species After Treatment with Anticancer Drugs
| Autor: | Alexander Erofeev, Christopher R. W. Edwards, Petr V. Gorelkin, Pavel Novák, Alexander G. Majouga, Anastasiia S Garanina, A. Alova, Natalia L. Klyachko, Helena Lopatukhina, Yanjun Zhang, Yasufumi Takahashi, Yuri E. Korchev, Stepan Vodopyanov, S. V. Salikhov, Maxim A. Abakumov, Alexander Vaneev, Oxana Ryabaya, Roman Akasov |
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| Rok vydání: | 2020 |
| Předmět: |
Cell
Antineoplastic Agents Biosensing Techniques Analytical Chemistry Mice In vivo Cell Line Tumor medicine Animals Humans Doxorubicin chemistry.chemical_classification Reactive oxygen species Chemistry Electrochemical Techniques Neoplasms Experimental In vitro medicine.anatomical_structure Mechanism of action Cell culture PC-3 Cells Biophysics medicine.symptom Reactive Oxygen Species Intracellular medicine.drug |
| Zdroj: | Analytical Chemistry. 92:8010-8014 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | In vivo monitoring of reactive oxygen species (ROS) in tumors during treatment with anticancer therapy is important for understanding the mechanism of action and in the design of new anticancer drugs. In this work, a platinized nanoelectrode is placed into a single cell for detection of the ROS signal, and drug-induced ROS production is then recorded. The main advantages of this method are the short incubation time with the drug and its high sensitivity which allows the detection of low intracellular ROS concentrations. We have shown that our new method can measure the ROS response to chemotherapy in tumor-bearing mice in real-time. ROS levels were measured in vivo inside the tumor at different depths in response to doxorubicin. This work provides an effective new approach for the measurement of intracellular ROS by platinized nanoelectrodes. |
| Databáze: | OpenAIRE |
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