Mechanical force antagonizes the inhibitory effects of RecX on RecA filament formation in Mycobacterium tuberculosis
| Autor: | Hu Chen, Kalappa Muniyappa, Xinghua Zhang, Shimin Le, Jin Chen, K. Neelakanteshwar Patil, Jie Yan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Magnetic tweezers
DNA repair DNA Single-Stranded macromolecular substances Mycobacterium tuberculosis Biology Genome Integrity Repair and Replication Molecular biology Biochemistry Nucleoprotein Biomechanical Phenomena Polymerization Protein filament chemistry.chemical_compound Rec A Recombinases Adenosine Triphosphate chemistry Bacterial Proteins ATP hydrolysis Genetics Biophysics Mechanosensitive channels Homologous recombination DNA |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Efficient bacterial recombinational DNA repair involves rapid cycles of RecA filament assembly and disassembly. The RecX protein plays a crucial inhibitory role in RecA filament formation and stability. As the broken ends of DNA are tethered during homologous search, RecA filaments assembled at the ends are likely subject to force. In this work, we investigated the interplay between RecX and force on RecA filament formation and stability. Using magnetic tweezers, at single molecular level, we found that Mycobacterium tuberculosis (Mt) RecX could catalyze stepwise de-polymerization of preformed MtRecA filament in the presence of ATP hydrolysis at low forces ( |
| Databáze: | OpenAIRE |
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