Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons

Autor: Volodymyr Krotov, Nana Voitenko, Boris V. Safronov, Pavel V. Belan, D. P. Shevchuk, K. S. Agashkov, M. Krasniakova, Y. Andrianov, Y. Zabenko
Přispěvatelé: Instituto de Investigação e Inovação em Saúde
Rok vydání: 2019
Předmět:
Nociception
0301 basic medicine
Lamina
Nerve Fibers
Unmyelinated / physiology
Population
Pain
Action Potentials
lcsh:Medicine
Stimulus (physiology)
Biology
Article
Neurons / physiology
03 medical and health sciences
0302 clinical medicine
Excitatory Postsynaptic Potentials / physiology
Afferent
Synapses / physiology
medicine
Animals
Rats
Wistar
lcsh:Science
education
Neurons
Nerve Fibers
Unmyelinated
education.field_of_study
Signal processing
Multidisciplinary
lcsh:R
Nociception / physiology
Excitatory Postsynaptic Potentials
Spinal cord
Cellular neuroscience
Rats
030104 developmental biology
medicine.anatomical_structure
Spinal Cord
Spinal Cord / cytology
Receptive field
Synapses
Spinal Cord / physiology
lcsh:Q
Neuroscience
Neurons / cytology
030217 neurology & neurosurgery
Action Potentials / physiology
Zdroj: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
ISSN: 2045-2322
Popis: Lamina I spino-parabrachial neurons (SPNs) receive peripheral nociceptive input, process it and transmit to the supraspinal centres. Although responses of SPNs to cutaneous receptive field stimulations have been intensively studied, the mechanisms of signal processing in these neurons are poorly understood. Therefore, we used an ex-vivo spinal cord preparation to examine synaptic and cellular mechanisms determining specific input-output characteristics of the neurons. The vast majority of the SPNs received a few direct nociceptive C-fiber inputs and generated one spike in response to saturating afferent stimulation, thus functioning as simple transducers of painful stimulus. However, 69% of afferent stimulation-induced action potentials in the entire SPN population originated from a small fraction (19%) of high-output neurons. These neurons received a larger number of direct Ad- and C-fiber inputs, generated intrinsic bursts and efficiently integrated a local network activity via NMDA-receptor-dependent mechanisms. The high-output SPNs amplified and integrated the nociceptive input gradually encoding its intensity into the number of generated spikes. Thus, different mechanisms of signal processing allow lamina I SPNs to play distinct roles in nociception. The authors thank Mr. Andrew Dromaretsky for the technical assistance. P.B. was supported by the National Academy of Sciences of Ukraine (NASU), grant NASU # 0116U004470, grant NASU#67/15-Н. N.V. was supported by the NASU Biotechnology and NASU-KNU grants; NIH 1R01NS113189-01. B.V.S. was supported by the FEDER funds through the COMPETE 2020 (POCI), Portugal 2020, and by the FCT project PTDC/NEU-NMC/1259/2014 (POCI-01-0145-FEDER-016588)
Databáze: OpenAIRE
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