Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression
| Autor: | Wei Zhao, Hong-Yu Zhao, Xue-Feng Gu, Jin-Yun Song, Qinghua Ji, Peng Zeng, Yiqiong Yang, Duo Shen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
miRNA sponge medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Medicine MMP19 business.industry Competing endogenous RNA Cell growth Gastroenterology Cancer medicine.disease Real-time polymerase chain reaction Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research 030211 gastroenterology & hepatology Original Article business Carcinogenesis Gastric cancer CircRNAs |
| Zdroj: | Journal of Gastric Cancer |
| ISSN: | 2093-5641 2093-582X |
| Popis: | Purpose Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. Materials and methods Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. Results The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. Conclusions Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism. |
| Databáze: | OpenAIRE |
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