One-step synthesis of glycogen-type polysaccharides from maltooctaose and its structural characteristics

Autor:	Phuong Lan Tran, Yan An, Goo-Yeong Jeong, So-Young Ban, Phu Cuong Nguyen, Euijeon Woo, SangGuan You, Jong-Tae Park
Rok vydání:	2022
Předmět:	Polymers and Plastics Organic Chemistry Materials Chemistry
Zdroj:	Carbohydrate Polymers. 284:119175
ISSN:	0144-8617
DOI:	10.1016/j.carbpol.2022.119175
Popis:	The one-step synthesis of glycogen-type polysaccharides from maltooctaose (G8) was accomplished based on the new findings of the catalytic mechanism of glycogen branching enzymes (GBEs) from Vibrio vulnificus, Deinococcus geothermalis, and Escherichia coli. GBEs from D. geothermalis and E. coli used maltononaose and maltotridecaose as the minimum, respectively, while V. vulnificus GBE (VvGBE) catalyzed the surprisingly small substrate, G8, into polysaccharides. Intriguingly, all three GBEs catalyzed α-1,4-transglycosylation at the early reaction stage of transglycosylation. VvGBE successfully converted the smallest substrate (G8) into two highly branched polysaccharides (HBP), in which the big polysaccharide (1.49 × 10
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01dc397b4ca57b218461faa3e5b50ff0 https://doi.org/10.1016/j.carbpol.2022.119175 Zobrazit plný text záznamu Full Text from ScienceDirect