The influence of hemolymph-binding protein on juvenile hormone stability and distribution in Manduca sexta fat body and imaginal discs in vitro
| Autor: | Lawrence I. Gilbert, J. Nowock, Walter Gooddman, Bruce D. Hammock, Vassiliki Stamoudis |
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| Rok vydání: | 1975 |
| Předmět: |
animal structures
Receptors Cell Surface Moths Biochemistry Carboxylesterase Endocrinology Drug Stability Hemolymph Animals Wings Animal Molecular Biology Epoxide Hydrolases chemistry.chemical_classification biology Binding protein fungi Esterases biology.organism_classification Juvenile-hormone esterase activity Juvenile Hormones Lepidoptera Kinetics Imaginal disc Enzyme Adipose Tissue chemistry Manduca sexta Larva Juvenile hormone Protein Binding Subcellular Fractions |
| Zdroj: | Molecular and Cellular Endocrinology. 3:167-184 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/0303-7207(75)90043-x |
| Popis: | The wing discs and fat body of Manduca sexta larvae contain enzymes (i.e. carboxylesterase and epoxide hydratase) that can convert the C 18 juvenile hormone (JH) to the acid, diol and acid diol. No evidence of oxidative degradation was noted. In vitro studies suggest that JH can be compartmentalized within the cells of the fat body where it is less accessible to degradative mechanisms. Experiments utilizing a hemolymph-binding protein fraction (BPF) in vitro with fat body and imaginal discs indicate that the BPF retards the uptake of JH by tissues and its subsequent degradation by tissue enzymes. BPF also appears to protect JH from degradation by enzymes released into the medium. By these mechanisms the insect can maintain elevated JH titers for relatively long periods. Binding protein may also keep JH in solution in the hemolymph allowing its rapid distribution throughout the insect. The data suggest that the binding protein plays a key role in maintaining juvenile hormone titers. |
| Databáze: | OpenAIRE |
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