Telomere reduction in endometrial adenocarcinoma
| Autor: | George Yeh, Julie K. Smith |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Genome instability
Pathology medicine.medical_specialty medicine.medical_treatment Molecular Sequence Data Biology Adenocarcinoma Endometrium medicine.disease_cause chemistry.chemical_compound medicine Carcinoma Tumor Cells Cultured Humans Deoxyribonucleases Type II Site-Specific Hysterectomy Base Sequence Obstetrics and Gynecology Nucleic Acid Hybridization DNA Neoplasm Telomere medicine.disease Endometrial Neoplasms medicine.anatomical_structure chemistry Cancer research Autoradiography Female Carcinogenesis Oligonucleotide Probes DNA |
| Zdroj: | American journal of obstetrics and gynecology. 167(6) |
| ISSN: | 0002-9378 |
| Popis: | OBJECTIVE: Some of the genomic instability that is observed in solid tumors may be due to the loss of telomeric sequences. These experiments were designed to compare the number of telomeric repeat sequences in endometrial adenocarcinoma with that found in adjacent normal tissue. STUDY DESIGN: Deoxyribonucleic acid was extracted from normal and malignant uterine tissue of 11 patients undergoing hysterectomy for treatment of endometrial adenocarcinoma and also from five endometrial carcinoma cell lines. The relative number of telomeric repeat sequences in each sample was measured by hybridization of these deoxyribonucleic acids to a probe specific for the human telomeric repeat. Hybridization signals were quantified by autoradiography and a β-particle detection system. RESULTS: A reduction of telomeric repeat sequences in tumor versus normal tissue was found in 10 of 11 cases. Telomere reduction was also seen in endometrial carcinoma cell lines. CONCLUSIONS: Telomere reduction is a genetic characteristic of many endometrial tumors. Telomere reduction may contribute to the genesiS and progression of endometrial carcinoma, or it may be a secondary effect of the tumorigenesis process. (AM J OBSTET GYNECOL 1992;167:1883-7.) |
| Databáze: | OpenAIRE |
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