Phenotypic and Functional Comparison of Mesenchymal Stem Cells Derived from the Bone Marrow of Normal Adults and Patients with Hematologic Malignant Diseases
Autor: | Kai Li, Zhi Chao Chen, Zhi Gang Zhao, Ping Zou, Yan Liang, Weiming Li, Qiu Bai Li |
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Rok vydání: | 2007 |
Předmět: |
Adult
Male Adolescent Cell Culture Techniques Bone Marrow Cells Biology Reference Values hemic and lymphatic diseases medicine Humans Stem cell transplantation for articular cartilage repair Reverse Transcriptase Polymerase Chain Reaction Lymphoma Non-Hodgkin Mesenchymal stem cell Myeloid leukemia Mesenchymal Stem Cells Cell Biology Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Hodgkin Disease Immunohistochemistry Hematopoiesis Lymphoma Haematopoiesis Phenotype medicine.anatomical_structure Cell culture Hematologic Neoplasms Immunology Cancer research Female Bone marrow Stem cell Cell Division Developmental Biology |
Zdroj: | Stem Cells and Development. 16:637-648 |
ISSN: | 1557-8534 1547-3287 |
Popis: | Mesenchymal stem cells (MSCs) have received much attention for their ability to differentiate into various cell types under specific conditions and to support the proliferation of hematopoietic stem cells. However, it is unclear whether the characteristics of MSCs are altered in different disease states. In this study, we obtained and expanded MSCs from bone marrow of patients with acute lymphoblastic leukemia (ALL), Hodgkin disease (HD), and non-Hodgkin lymphoma (NHL). Our results showed that MSCs derived from ALL, HD, and NHL were similar to normal adult bone marrow-derived MSCs in morphology, growth properties, surface epitopes, and differentiation ability in vitro. Moreover, MSCs derived from ALL, NHL, and HD had a normal karyotype and ultrastructure. These cells could express hematopoietic cytokines and support hematopoiesis in long-term culture. However, adherent cells isolated from bone marrow of patients with acute myeloid leukemia (AML) showed abnormal biological properties, including heterogeneity in morphology, limited proliferation capacity, and impaired differentiation and hematopoiesis support ability. These results indicate that there are differences in the characteristics of adherent cells derived from different disease states, which may be important for reasonable MSC selection in stem cell-based therapy. |
Databáze: | OpenAIRE |
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