Low expression of the interleukin (IL)-4 receptor alpha chain and reduced signalling via the IL-4 receptor complex in human neonatal B cells
| Autor: | Kevin M. Dischert, James E. Crowe, James N. Higginbotham, Cuixia Tian, Grace K. Kron |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Chemokine Immunology Naive B cell Gene Expression Apoptosis Cell Separation Interleukin-4 receptor Humans Immunology and Allergy Phosphorylation Receptor Analysis of Variance B-Lymphocytes Interleukin-13 biology Reverse Transcriptase Polymerase Chain Reaction Infant Newborn Interleukin-4 Receptor alpha Subunit Interleukin Original Articles Fetal Blood Flow Cytometry Interleukin-13 Receptor alpha1 Subunit Molecular biology Interleukin 10 biology.protein Interleukin-4 STAT6 Transcription Factor Alpha chain Signal Transduction |
| Zdroj: | Immunology. 119:54-62 |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1111/j.1365-2567.2006.02405.x |
| Popis: | Diminished neonatal antibody responses following infection or immunization may stem in part from intrinsic characteristics of neonatal B cells. In this study, we used B-cell subset sorting combined with gene expression assays to investigate major differences in the expression of host genes in neonatal and adult naïve B cells. We discovered significantly reduced expression of the interleukin (IL)-4 receptor alpha chain and reduced IL-4-induced signalling in neonatal B cells. Neonatal naïve B cells were susceptible to more rapid and more profound levels of apoptosis when cultured in vitro. They also exhibited a limited response to IL-4 treatment compared with adult cells. The expression level of the IL-13 receptor alpha 1 chain, a key component of the IL-13 receptor/IL-4 type II receptor, and the response to IL-13 treatment for protection against apoptosis in neonatal B cells were similar to those of the adult B cells. These studies suggest a possible mechanism underlying the limited magnitude and durability of neonatal antibody responses. |
| Databáze: | OpenAIRE |
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