Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion
| Autor: | Silvina Ghio, Paola Talia, Ornella Mailén Ontañon, Mercedes Maria Garrido, Eleonora Campos, María B. Bradanini, Rubén Marrero Díaz de Villegas, Florencia Elizabeth Piccinni |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
BETA-GLUCOSIDASE Bioethanol Production Bioconversion ENDOGLUCANSE lcsh:Biotechnology Cellobiose Cellulase Beta-glucan Polysaccharide Saccharification 01 natural sciences Applied Microbiology and Biotechnology Beta-glucosidase 03 medical and health sciences chemistry.chemical_compound Paenibacillus Hydrolysis SACCHARIFICATION 010608 biotechnology lcsh:TP248.13-248.65 Hidrólisis Endoglucanse Food science Glucans 030304 developmental biology chemistry.chemical_classification Glucanos 0303 health sciences Beta-glucanos biology GH1 GH8 biology.organism_classification Beta-glucans chemistry purl.org/becyt/ford/4.1 [https] biology.protein Fermentation BETA-GLUCAN Producción de Bioetanol purl.org/becyt/ford/4 [https] Biotechnology Research Article |
| Zdroj: | Biotechnology Reports, Vol 28, Iss, Pp e00526-(2020) Biotechnology Reports 28 : e00526 (Diciembre 2020) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Biotechnology Reports |
| Popis: | Highlights • Cel8Pa is an extracellular, halotolerant, broad substrate endoglucanase. • Bg1Pa is an intracellular β-glucosidase, with activity on cello oligosaccharides and high resistance to ethanol. • The concerted action of Cel8Pa and Bg1Pa has a synergistic effect on saccharification of β-glucans. • Cel8Pa and Bg1Pa are cold-stable and candidates for SSF ethanol 2 G processes. In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from Paenibacillus xylanivorans A59. Cel8Pa was active on a broad range of substrates, such as β-glucan from barley (24.5 IU/mg), lichenan (17.9 IU/mg), phosphoric acid swollen cellulose (PASC) (9.7 IU/mg), carboxi-methylcellulose (CMC) (7.3 IU/mg), chitosan (1.4 IU/mg) and xylan (0.4 IU/mg). Bg1Pa was active on cellobiose (C2) and cello-oligosaccharides up to C6, releasing glucose as the main product. When both enzymes were used jointly, there was a synergic effect in the conversion rate of polysaccharides to glucose. Cel8Pa and Bg1Pa presented important properties for simultaneous saccharification and fermentation (SSF) processes in second generation bioethanol production, such as tolerance to high concentration of glucose and ethanol. |
| Databáze: | OpenAIRE |
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