In vitro evaluation of antibacterial activities and anti-inflammatory effects of Bifidobacterium spp. addressing acne vulgaris
| Autor: | Nam-Joo Ha, Mi Jin Kim, Kang-Oh Lee, Hyang-Mi An, Si Won Lee, Kyungjae Kim, Cheolin Park, Seon-Hee Shin, Do-Kyung Lee, Min-Kyung Cha, Ji-Wook Ham |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Staphylococcus aureus medicine.drug_class Microbial Sensitivity Tests medicine.disease_cause Nitric Oxide Anti-inflammatory law.invention Microbiology Cell Line Probiotic Propionibacterium acnes Mice Sonication fluids and secretions law Disk Diffusion Antimicrobial Tests Drug Discovery Acne Vulgaris Antibiosis medicine Animals Acne Bifidobacterium Microbial Viability biology Tumor Necrosis Factor-alpha Macrophages Organic Chemistry Antimicrobial biology.organism_classification medicine.disease Anti-Bacterial Agents Molecular Medicine Inflammation Mediators Antibacterial activity |
| Zdroj: | Archives of pharmacal research. 35(6) |
| ISSN: | 1976-3786 |
| Popis: | The objective of this study was to evaluate the antibacterial and anti-inflammatory effect of Bifidobacterium spp. In the first part of the study, the antibacterial activities of live and sonicated cells, from a total of 23 Bifidobacterium species, on the growth of 5 different strain of Staphylococcus aureus. Six strains, of sonicated Bifidobacterium, exhibited antibacterial activity against staphylococci samples, and seven Bifidobacterium strains exhibited antibacterial activity on the growth of S. aureus S.P.-N2. In the second part of the study, we tested the antimicrobial activity, of Bifidobacterium against Propionibacterium acne KCTC3320, using the co-culture method. The loss of P. acnes viability, caused by B. adolescentis SPM0308 and B. longum SPM1207, was 84% and 75%, respectively (*p < 0.05). In the third part of the study, the anti-inflammatory activity of B. adolescentis SPM0308 and B. longum SPM1207 were assessed; nitric oxide (NO), and tumor necrosis factor-α (TNF-α), production were tested using the murine macrophage RAW 264.7 cell line. Treatment of RAW 264.7 cells, with Bifidobacterium, decreased production of NO and TNF-α rather than LPS (100 ng/mL) treatment. The results suggest that B. adolescentis SPM0308 could be used as an effective control for P. acnes KCTC3320, and S. aureus, and reduce the risk of acne vulgaris development. We suggest that B. adolescentis SPM0308 may be a useful probiotic microorganism, for prevention of acne vulgaris, without adverse effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink