The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers
| Autor: | Norman Henry Carey, S.M. Doel, A. G. Stewart, T. Cartwright, Michael Houghton, J.R. Birch, M.A.W. Eaton, H. M. Lewis, John C. Smith, John Spencer Emtage, Alan George Porter, T.P. Patel |
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| Rok vydání: | 1980 |
| Předmět: |
Signal peptide
chemistry.chemical_classification education.field_of_study Poly T Oligonucleotide Population Genes MHC Class II Oligonucleotides RNA-Directed DNA Polymerase Biology Fibroblasts Molecular biology Reverse transcriptase Amino acid chemistry Oligodeoxyribonucleotides Complementary DNA Genetics Coding region Humans Amino Acid Sequence Interferons RNA Messenger education Peptide sequence |
| Zdroj: | Nucleic acids research. 8(9) |
| ISSN: | 0305-1048 |
| Popis: | From recently published data on the amino-terminal structures of human and mouse interferons, we have predicted and synthesised an oligonucleotide capable of priming specifically the reverse transcription of human fibroblast interferon mRNA present within a total mRNA population. From these transcripts we determined the sequence of the 5'-terminus of the mRNA and identified a putative pre-peptide signal sequence. This enabled us to predict the sequence of another primer capable of directing the synthesis of interferon double-stranded cDNA corresponding to the entire coding region of the mRNA. Further sequencing studies also enabled us to establish the identity of 47 consecutive amino acids beginning with the methionine residue at the amino-terminus of the mature protein. |
| Databáze: | OpenAIRE |
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