Assessment of DNA interstrand crosslinks in NIH/3T3 cells induced by Chloroethylnitrosoureas
| Autor: | Minjun Guo, Xiaotong Zheng, Rugang Zhong, Lin-Na Zhao, Xuechai Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Drug
Environmental Engineering media_common.quotation_subject lcsh:QR1-502 Biology Industrial and Manufacturing Engineering 3T3 cells lcsh:Microbiology lcsh:Physiology chemistry.chemical_compound lcsh:Zoology medicine lcsh:QL1-991 Cytotoxicity media_common Carmustine lcsh:QP1-981 Nimustine Semustine Molecular biology medicine.anatomical_structure chemistry Chemical engineering Secondary tumors DNA medicine.drug |
| Zdroj: | BIO Web of Conferences, Vol 8, p 01019 (2017) |
| ISSN: | 2117-4458 |
| Popis: | Aim: To investigated the mechanism of Chloroethylnitrosoureas (CENUs) leading to secondary tumors after therapy, three kinds of CENUs, namely, Nimustine (ACNU), Carmustine (BCNU) and Semustine (MeCCNU) were used to investigated drug-induced DNA interstrand crosslinks(ICLs). Method: The alkaline comet assay was adopted to compare DNA damages induced by CENUs under different concentrations. Results: With the increase of drug’s concentration, DNA migration exhibited a positive concentration-dependent relationship in all three drugs. ACNU was shown to cause significant crosslinking. The other two drugs also induced crosslinking, but this could not be analyzed at high concentrations due to the high cytotoxicity of the drugs which caused cells death. |
| Databáze: | OpenAIRE |
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