Simultaneous quantification of cryptotanshinone and its active metabolite tanshinone IIA in plasma by liquid chromatography/tandem mass spectrometry (LC-MS/MS)
| Autor: | Haiping Hao, Peng Li, Zuoqi Ding, Guangji Wang, Jing Li |
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| Přispěvatelé: | Hao, Haiping, Wang, Guangji, Li, Peng, Ding, Zuoqi |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Metabolite Electrospray ionization Clinical Biochemistry Ethyl acetate Pharmaceutical Science Mass spectrometry Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Drug Stability Liquid chromatography–mass spectrometry Drug Discovery Animals Spectroscopy Active metabolite Chromatography High Pressure Liquid Chromatography Selected reaction monitoring Reproducibility of Results Pharmacology and Pharmaceutical Sciences Phenanthrenes Triple quadrupole mass spectrometer Rats chemistry Abietanes Drugs Chinese Herbal |
| Popis: | A rapid and sensitive method for the simultaneous determination of cryptotanshinone and its active metabolite tanshinone IIA in rat plasma was developed and well validated, using high-performance liquid chromatographic separation with tandem mass spectrometric detection. This method entailed a single step of liquid-liquid extraction with ethyl acetate from a small volume of plasmas. The analytes and internal standard diazepam were baseline separated on a Shim-pack VP-ODS analytical column. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated under selected reaction monitoring (SRM) mode. The method was linear in the concentration range of 1-100 ng/ml for both tanshinone IIA and cryptotanshinone. The intra- and inter-day precisions (R.S.D.%) were within 10.2% for both analytes. Deviation of the assay accuracies was within +/-12.0% for both analytes. Both analytes were proved to be stable during all sample storing, preparation and analytic procedures. The method was successfully applied to a pharmacokinetic study after an oral administration of cryptotanshinone to rats with a dose of 20 mg/kg. With the lower limits of quantification at 1.0 ng/ml for tanshinone IIA and 0.2 ng/ml for cryptotanshinone, this method was proved to be sensitive enough and reproducible for the pharmacokinetics study of both tanshinones. |
| Databáze: | OpenAIRE |
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