Real‐time quantitative PCR : A tool for absolute and relative quantification

Autor:	Ravikumar Harshitha, Duraipandian Rex Arunraj
Rok vydání:	2021
Předmět:	Science instruction Computer science Absolute quantification Real-Time Polymerase Chain Reaction computer.software_genre Biochemistry Melting curve analysis Real-time polymerase chain reaction Quantitative Real Time PCR Data mining Laboratory experiment Molecular Biology computer Software
Zdroj:	Biochemistry and Molecular Biology Education. 49:800-812
ISSN:	1539-3429 1470-8175
Popis:	Real-time quantitative PCR is a technique used to monitor the PCR reaction in real time. RT-qPCR is broadly classified into two types based on its purpose: absolute and relative quantification. Absolute quantification is used in a wide array of fields such as microbiology, food technology, and biotechnology to quantify the microbiological load/adulterants in a commodity/copy numbers respectively, whereas Relative quantification is used in the field of genomics and functional transcriptomics to perform gene expression analysis in biological experiments. A laboratory work that covers the basic principles involved in RT-qPCR and data analysis using the manual as well as the software methods are incorporated. The laboratory experiment was designed to provide insights on certain important principles such as primer characteristics, PCR efficiency, and melt curve analysis. This laboratory exercise provides all the significant components of RT-qPCR, which can be useful while performing an experiment in an undergraduate and graduate laboratory.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01cfa46f5a61b700a739c87eb6857729 https://doi.org/10.1002/bmb.21552 Zobrazit plný text záznamu Plný text