Comparative Strain Analysis ofAnaplasma phagocytophilumInfection and Clinical Outcomes in a Canine Model of Granulocytic Anaplasmosis
| Autor: | Kristen C. DeBisceglie, Diana G. Scorpio, Judith A. Cook, Nicole C. Barat, Melissa J. Beall, Brett A. Stillman, J. Stephen Dumler, Christopher E. Barat, Ramaswamy Chandrashekar |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Anaplasmosis Neutrophils animal diseases HL-60 Cells Pilot Projects Real-Time Polymerase Chain Reaction Microbiology Serology Dogs Virology parasitic diseases medicine Animals Humans Anaplasma Lymphatic Diseases Leukopenia Cell-Free System biology medicine.diagnostic_test Complete blood count bacterial infections and mycoses biology.organism_classification medicine.disease Antibodies Bacterial Anaplasma phagocytophilum Bacterial Load Specific Pathogen-Free Organisms Disease Models Animal Infectious Diseases Injections Intravenous Immunology biology.protein Cytokines medicine.symptom Antibody Generalized lymphadenopathy Granulocytes |
| Zdroj: | Vector-Borne and Zoonotic Diseases. 11:223-229 |
| ISSN: | 1557-7759 1530-3667 |
| Popis: | A pilot study was conducted to determine whether existing human or canine strains of Anaplasma phagocytophilum would reproduce clinical disease in experimentally inoculated dogs similar to dogs with naturally acquired granulocytic anaplasmosis. Six hounds were inoculated intravenously with one human and two canine strains of A. phagocytophilum that were propagated in vitro in HL-60 cells or in infected autologous neutrophils. Infected dogs were monitored for lethargy, anorexia, petechiae, lymphadenopathy, and fever. Dogs were assessed for complete blood count (CBC), serum chemistry, and serology (IFA and SNAP® 4Dx®); for A. phagocytophilum blood load by quantitative polymerase chain reaction; and for cytokine production. Prominent clinical signs were generalized lymphadenopathy and scleral injection; only one dog developed fever lasting 4 days. Notable laboratory alterations included sustained leukopenia and thrombocytopenia in all dogs. A. phagocytophilum morulae were noted in blood between days 10 and 11, although all dogs retained A. phagocytophilum DNA in blood through day 60. All dogs seroconverted by days 10-15 by IFA, and by days 17-30 by SNAP 4Dx; cytokine analyses revealed 10-fold increases in interleukin-2 and interleukin-18 in the neutrophil-propagated 98E4 strain-infected dog. All A. phagocytophilum strains produced infection, although canine 98E4 strain reproduced clinical signs, hematologic changes, and inflammatory cytokine elevations most consistent with granulocytic anaplasmosis when recognized clinically. Therefore, this strain should be considered for use in future studies of A. phagocytophilum canine infection models. |
| Databáze: | OpenAIRE |
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