Occurrence of 5'-deoxyadenosylcobalamin and its physiological function as the coenzyme of methylmalonyl-CoA mutase in a marine eukaryotic microorganism, Schizochytrium limacinum SR21
| Autor: | Masahiro Hayashi, Yoshihisa Nakano, Emi Miyamoto, Tsugiyo Yukino, Fumio Watanabe, Yuri Tanioka |
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| Rok vydání: | 2008 |
| Předmět: |
Coenzymes
Medicine (miscellaneous) Cobalamin Cofactor Microbiology chemistry.chemical_compound Mutase Animals Cyanocobalamin Schizochytrium limacinum Nutrition and Dietetics biology Fatty acid metabolism Methylmalonyl-CoA mutase Fatty Acids Temperature Eukaryota Methylmalonyl-CoA Mutase biology.organism_classification Enzyme Activation Molecular Weight B vitamins Biochemistry chemistry biology.protein Cobamides |
| Zdroj: | Journal of nutritional science and vitaminology. 53(6) |
| ISSN: | 0301-4800 |
| Popis: | A marine eukaryotic microorganism, Schizochytrium limacinum SR21, had the ability to absorb and accumulate exogenous cobalamin, which was converted to the cobalamin coenzymes 5'-deoxyadenosylcobalamin (20.1%) and methylcobalamin (29.6%). A considerably high activity (about 38 mU/mg protein) of 5'-deoxyadenosylcobalamin-dependent methylmalonyl-CoA mutase (EC 5.4.99.2) involved in amino acid and odd-chain fatty acid metabolism was found in the cell homogenate of S. limacinum SR21. The enzyme was purified to homogeneity and characterized. |
| Databáze: | OpenAIRE |
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