Análise filogenética de Phytophthora capsici Leonian do Estado de São Paulo baseada na seqüência de nucleotídeos da região ITS-5.8S rDNA
| Autor: | Janaína M. Marque, Daniel Dias Rosa, Nilton Luiz de Souza, Roseli Chela Fenille, Eiko E. Kuramae |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Mapa, Fungal Biodiversity Center |
| Rok vydání: | 2003 |
| Předmět: |
Phytophthora
polymerase chain reaction ribosome DNA phylogeny General Biochemistry Genetics and Molecular Biology Leonia Phylogenetics evolution genetic variability parasitic diseases Botany Genetic variation Phytophthora capsici Genetic variability Internal transcribed spacer Phytophthora sp Ribosomal DNA Genetics nonhuman biology Phylogenetic tree nucleotide sequence biology.organism_classification internal transcribed spacer ITS General Agricultural and Biological Sciences Brazil |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1807-863X 1679-9283 2344-4452 |
| DOI: | 10.4025/actascibiolsci.v25i2.2033 |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:20:41Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:28:59Z : No. of bitstreams: 1 2-s2.0-23444452895.pdf: 246833 bytes, checksum: fbd7512d4c883ab6ed7a179a8399dc34 (MD5) Made available in DSpace on 2014-05-27T11:20:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-07-01 Phylogenetic analysis through morphological and cultural traits is considered inconsistent and hard to be scientifically accepted once there is no way to establish the direction of evolution on morphological traits. Molecular markers are suitable for phylogenetic analysis. The sequencing of ITS1 and ITS2 (internal transcribed spacers) regions and of 5.8S gene from ribosomal DNA were used to estimate the genetic variation and distance of 10 Phytophthora capsici isolates. The amount of genetic variation amongst isolated P. capsici from distinct regions of São Paulo State was 0.1 to 1.6 and 0.1 to 1.1% at ITS1 and ITS2, respectively, and a phylogenetic distance of about 78.5% between P. capsici and Phytophthora spp. was observed. Departamento de Produção Vegetal Universidade Estadual Paulista, Caixa Postal 237, 18603-970, Botucatu, São Paulo Laboratório de Apoio Vegetal Mapa, Pra̧a Cívica, 100, 74003-010, Goiania, Goias Centraalbureau Voor Schimmelcultures Fungal Biodiversity Center, Utrecht Departamento de Produção Vegetal Universidade Estadual Paulista, Caixa Postal 237, 18603-970, Botucatu, São Paulo |
| Databáze: | OpenAIRE |
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