Imaging beyond the super-resolution limits using ultrastructure expansion microscopy (UltraExM)
| Autor: | Marketa Cernohorska, Paul Guichard, Jan-Gero Schloetel, Susanne Borgers, Edward S. Boyden, Fabian U. Zwettler, Davide Gambarotto, Markus Sauer, Michael Unser, Jörn Heine, Matthias Reuss, Virginie Hamel, Denis Fortun |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
Materials science Resolution (electron density) law.invention 03 medical and health sciences 0302 clinical medicine Optical microscope law Organelle Microscopy Biophysics Fluorescence microscope Ultrastructure Electron microscope Image resolution 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/308270 |
| Popis: | For decades, electron microscopy (EM) was the only method able to reveal the ultrastructure of cellular organelles and molecular complexes because of the diffraction limit of optical microscopy. In recent past, the emergence of superresolution fluorescence microscopy enabled the visualization of cellular structures with so far unmatched spatial resolution approaching virtually molecular dimensions. Despite these technological advances, currently super-resolution microscopy does not permit the same resolution level as provided by electron microscopy, impeding the attribution of a protein to an ultrastructural element. Here, we report a novel method of near-native expansion microscopy (UltraExM), enabling the visualization of preserved ultrastructures of macromolecular assemblies with subdiffraction-resolution by standard optical microscopy. UltraExM revealed for the first time the ultrastructural localization of tubulin glutamylation in centrioles. Combined with super-resolution microscopy, UltraExM unveiled the centriolar chirality, an ultrastructural signature, which was only visualizable by electron microscopy. |
| Databáze: | OpenAIRE |
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