Hyperediting of human T-cell leukemia virus type 2 and simian T-cell leukemia virus type 3 by the dsRNA adenosine deaminase ADAR-1

Autor: Nga Ling Ko, Jean-Pierre Vartanian, Renaud Mahieux, Emmanuel Birlouez, Simon Wain-Hobson
Přispěvatelé: Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Rétrovirologie Moléculaire, Institut Pasteur [Paris] (IP), Oncogenèse rétrovirale – Retroviral Oncogenesis (OR), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by funds from the Institut Pasteur the CNRS and the DGA (Direction Générale des Armées). The Molecular Retrovirology Unit is ‘Equipe labellisée LIGUE 2010’. R. M. is supported by Ecole Normale Supérieure de Lyon, by INSERM and through funding from the Programme interdisciplinaire CNRS Maladies infectieuses émergentes and from NIH (grant AI072495-01)., Vartanian, Jean-Pierre, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
MESH: Simian T-lymphotropic virus 3
Adenosine
MESH: Mutation
Adenosine Deaminase
viruses
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
T-cell leukemia
Viral transformation
Genome
Viral
MESH: Base Sequence
Polymerase Chain Reaction
MESH: Sequence Homology
Nucleic Acid
Virus
Adenosine deaminase
Sequence Homology
Nucleic Acid
Virology
Animals
Humans
MESH: RNA Editing
MESH: Animals
MESH: Adenosine Deaminase
Simian T-lymphotropic virus 3
MESH: Humans
MESH: Molecular Sequence Data
Base Sequence
biology
MESH: Human T-lymphotropic virus 2
Animal
Human T-lymphotropic virus 2
RNA-Binding Proteins
RNA
MESH: Polymerase Chain Reaction
MESH: Adenosine
Molecular biology
[SDV] Life Sciences [q-bio]
HEK293 Cells
MESH: RNA-Binding Proteins
RNA editing
MESH: HEK293 Cells
MESH: RNA
Viral
Mutation
ADAR
biology.protein
RNA
Viral
RNA Editing
MESH: Genome
Viral
Zdroj: Journal of General Virology
Journal of General Virology, 2012, 93 (12), pp.2646-2651. ⟨10.1099/vir.0.045146-0⟩
ISSN: 0022-1317
1465-2099
DOI: 10.1099/vir.0.045146-0⟩
Popis: RNA editing mediated by adenosine deaminases acting on RNA (ADARs) converts adenosine (A) to inosine (I) residues in dsRNA templates. While ADAR-1-mediated editing was essentially described for RNA viruses, the present work addresses the issue for two δ-retroviruses, human T-cell leukemia virus type 2 and simian T-cell leukemia virus type 3 (HTLV-2 and STLV-3). We examined whether ADAR-1 could edit HTLV-2 and STLV-3 virus genomes in cell culture andin vivo. Using a highly sensitive PCR-based method, referred to as 3DI-PCR, we showed that ADAR-1 could hypermutate adenosine residues in HTLV-2. STLV-3 hypermutation was obtained without using 3DI-PCR, suggesting a higher mutation frequency for this virus. Detailed analysis of the dinucleotide editing context showed preferences for 5′ ArA and 5′ UrA. In conclusion, the present observations demonstrate that ADAR-1 massively edits HTLV-2 and STLV-3 retrovirusesin vitro, but probably remains a rare phenomenonin vivo.
Databáze: OpenAIRE
Externí odkaz: