Atrial natriuretic peptide effect on NADPH-diaphorase in rat intestinal tract
| Autor: | M.C. Ortiz, M.P. Majowicz, María Angeles Costa, L.V. González Bosc, C. Arranz, A. M. Balaszczuk, Francisco Capani, J. Pecci Saavedra, N.A. Vidal, J. J. Lopez Costa |
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| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Colon Physiology Photoperiod Nitric Oxide Biochemistry Nitric oxide Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Atrial natriuretic peptide Internal medicine Intestine Small Image Processing Computer-Assisted medicine Animals Large intestine Intestinal Mucosa Rats Wistar Receptor Cyclic GMP Incubation biology Histocytochemistry NADPH Dehydrogenase Small intestine Rats Staining Isoenzymes Nitric oxide synthase NG-Nitroarginine Methyl Ester medicine.anatomical_structure chemistry Guanylate Cyclase cardiovascular system biology.protein Nitric Oxide Synthase Receptors Atrial Natriuretic Factor Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists circulatory and respiratory physiology |
| Zdroj: | Peptides. 20:615-621 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/s0196-9781(99)00015-7 |
| Popis: | Histochemical reaction of NADPH-diaphorase (NOS-NADPH-d) was used to identify NO synthesis. A 30-min 0.1 μg/kg/min ANP infusion led to about a 10% and 35% increase in small and large intestine enterocytes stain respectively. This increase was abolished by a bolus of 1 mg/kg l -NAME before ANP infusion in small intestine, and partially abolished it in colon. Incubation of small and large intestine with 0.5 μM ANP increased stain at about 20%. In both tissues the preincubation with 0.1 mM l -NAME abolished the ANP effect. Incubation with 0.1 mM 8-Br-cGMP enhanced staining about 70% and 30% in small and large intestine respectively. Our results show that ANP enhances NOS-NADPH-d activity, suggesting that ANP stimulates NO synthase in enterocytes by l -arginine-NO pathway. 8-Br-cGMP mimicked the effect of ANP described above. Therefore, the guanylate cyclase-coupled natriuretic receptors, NPR-A and NPR-B, probably mediate this ANP effect. |
| Databáze: | OpenAIRE |
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