Surfactant metabolism in surfactant protein A-deficient mice
| Autor: | Machiko Ikegami, T. R. Korfhagen, M. D. Bruno, Alan H. Jobe, J. A. Whitsett |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Pulmonary Surfactant-Associated Proteins Physiology Metabolic Clearance Rate Proteolipids Biology chemistry.chemical_compound Mice Pulmonary surfactant In vivo Physiology (medical) Phosphatidylcholine Internal medicine medicine Choline Animals Lung Pulmonary Surfactant-Associated Protein A Mice Knockout Sheep Body Weight Homozygote Pulmonary Surfactants Cell Biology Organ Size In vitro Surfactant protein A Endocrinology chemistry biology.protein Phosphatidylcholines Protein A |
| Zdroj: | The American journal of physiology. 272(3 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | In the present study we asked if surfactant metabolism was altered in surfactant protein (SP) A-deficient mice in vivo. Although previous studies in vitro demonstrated that SP-A modulates surfactant secretion and reuptake by type II cells, mice made SP-A deficient by homologous recombination grow and reproduce normally and have normal lung function. Alveolar and lung tissue saturated phophatidylcholine (Sat PC) pools were 50 and 26% larger, respectively, in SP-A(-/-) mice than in SP-A(+/+) mice. Radiolabeled choline and palmitate incorporation into lung Sat PC was similar both in vivo and for lung tissue slices in vitro from SP-A(+/+) and SP-A(-/-) mice. Percent secretion of radiolabeled Sat PC was unchanged from 3 to 15 h, although SP-A(-/-) mice retained more labeled Sat PC in the alveolar lavages at 48 h (consistent with the increased surfactant pool sizes). Clearance of radiolabeled dipalmitoylphosphatidylcholine and SP-B from the air spaces after intratracheal injection was similar in SP-A(-/-) and SP-A(+/+) mice. Lack of SP-A had minimal effects on the overall metabolism of Sat PC or SP-B in mice. |
| Databáze: | OpenAIRE |
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