A hybrid FIA/HPLC system incorporating monolithic column chromatography
| Autor: | Jacqui L. Adcock, Neil W. Barnett, Graham D. Marshall, Paul S. Francis, Kent M. Agg |
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| Rok vydání: | 2007 |
| Předmět: |
Flow injection analysis
Neurotransmitter Agents Chromatography Thebaine Monolithic HPLC column Molecular Structure Chemistry Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Alkaloids Column chromatography Oripavine Flow Injection Analysis medicine Humans Environmental Chemistry Amines Chromatography High Pressure Liquid Spectroscopy Pseudomorphine medicine.drug Opiate alkaloid |
| Zdroj: | Analytica Chimica Acta. 600:136-141 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2007.03.048 |
| Popis: | We have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5-hydroxyindole-3-acetic acid and homovanillic acid) in human urine, using tris(2,2'-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection. |
| Databáze: | OpenAIRE |
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