Sensitive detection of albuminuria by graphene oxide-mediated fluorescence quenching aptasensor
Autor: | Patcharee Isarankura-Na-Ayudhya, Preedee Pinpradup, Wireeya Chawjiraphan, Nuankanya Sathirapongsasuti, Prapasiri Pongprayoon, Khoonsake Segkhoonthod, Chayachon Apiwat, Deanpen Japrung, Kiatnida Treerattrakoon, Patraporn Luksirikul |
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Rok vydání: | 2020 |
Předmět: |
Aptamer
Oxide Serum Albumin Human Biosensing Techniques 02 engineering and technology Urine 010402 general chemistry 01 natural sciences Analytical Chemistry law.invention chemistry.chemical_compound Limit of Detection law medicine Albuminuria Humans Instrumentation Spectroscopy Detection limit Chromatography Graphene Albumin Aptamers Nucleotide 021001 nanoscience & nanotechnology Human serum albumin Atomic and Molecular Physics and Optics 0104 chemical sciences Spectrometry Fluorescence chemistry Graphite medicine.symptom 0210 nano-technology medicine.drug |
Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 231:118128 |
ISSN: | 1386-1425 |
Popis: | Albuminuria is a pathological condition wherein the human serum albumin (HSA) protein is present in abnormally excess amounts in the urine. A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albumin in urine samples and HSA in serum samples. The aptamer-bound HSA used in this aptasensor has hairpin structures, which are characteristic of the aptamer binding site. The limit of detection of the developed platform is 0.05 μg·mL−1 and the detection range is 0.1–14.0 μg·mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with kidney diseases. This approach can be modified to measure HSA using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for other standard automated methods. Therefore, this aptasensor has significant potential for commercialization and wide-scale public use. |
Databáze: | OpenAIRE |
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