Differences in the mechanisms of growth control in contact-inhibited and serum-deprived human fibroblasts
| Autor: | R.J. Wieser, Cornelia Dietrich, Katja Wallenfang, Franz Oesch |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Cell Cycle Proteins Protein Serine-Threonine Kinases Retinoblastoma Protein Culture Media Serum-Free S Phase Cyclin D1 Cyclins Proto-Oncogene Proteins CDC2-CDC28 Kinases Genetics medicine Humans Cyclin D3 Phosphorylation Fibroblast Molecular Biology Cyclin-Dependent Kinase Inhibitor p16 Cyclin biology Cell growth Tumor Suppressor Proteins Cyclin-Dependent Kinase 2 Cyclin-dependent kinase 2 G1 Phase Cyclin-Dependent Kinase 4 Fibroblasts Diploidy Cyclin-Dependent Kinases Culture Media Cell biology medicine.anatomical_structure Cell culture biology.protein biological phenomena cell phenomena and immunity Signal transduction Microtubule-Associated Proteins Cell Division Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | Oncogene. 15:2743-2747 |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | In the present work we studied mechanisms of growth control in contact-inhibited and serum-deprived human diploid fibroblasts. The observation that the effects on [3H]thymidine incorporation and reduction of retinoblastoma gene product-phosphorylation were additive when contact-inhibition and serum-deprivation were combined led us to the conclusion that the underlying mechanisms might be different. Both contact-inhibition and serum-deprivation led to a strong decrease of cdk4-kinase-activity and cdk2-phosphorylation at Thr 160, while the total amounts of cdk4 and cdk2 remained constant. In contact-inhibited cells, we revealed a strong protein accumulation of the cdk2-inhibitor p27 and a slight, but significant increase of the cdk4-inhibitor p16. In serum-deprived cells, the protein levels in p27 and p16 remained low. In contrast, we detected a rapid decrease of cyclin D1 and cyclin D3 which did not occur in contact-inhibited cells. These results indicate that serum-deprivation and contact-inhibition have different mechanisms although they affect the same pathway cyclin D – cdk4, pRB, cyclin E – cdk2. |
| Databáze: | OpenAIRE |
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