Effect of THP-1 Cells on the Formation of Vascular Tubes by Endothelial EA.hy926 Cells in the Presence of Placenta Secretory Products
| Autor: | T. Yu. L’vova, S. A. Selkov, K. L. Belyakova, D. I. Sokolov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Endothelium Angiogenesis Placenta Pregnancy Trimester Third Cell Neovascularization Physiologic Cell Communication Models Biological General Biochemistry Genetics and Molecular Biology Monocytes Preeclampsia Cell Line Tissue Culture Techniques 03 medical and health sciences 0302 clinical medicine Pre-Eclampsia Pregnancy medicine Humans THP1 cell line Chemistry Endothelial Cells General Medicine medicine.disease Coculture Techniques Cell biology Endothelial stem cell Pregnancy Trimester First 030104 developmental biology medicine.anatomical_structure Culture Media Conditioned embryonic structures Immunology Female 030217 neurology & neurosurgery Ea hy926 |
| Zdroj: | Bulletin of experimental biology and medicine. 162(4) |
| ISSN: | 1573-8221 |
| Popis: | We studied the effect of THP-1 cells on the formation of vessel-like structures by endothelial cells in the presence of placenta-conditioned media. Addition of THP-1 cells to endothelial cells cultured in the presence of media conditioned by first-trimester placentas led to an increase in the length of cell tubes and reduced their number in comparison with endothelial cell monoculture. In the presence of media conditioned by third-trimester placentas, THP-1 cells did not affect the length and number of cell tubes formed by endothelial cells. When evaluating the formation of vessel-like structures by endothelial cells in co-culture, marked decrease in the length of cell tubes in the presence of media conditioned by first-trimester placentas vs. third-trimester placentas was noted. No differences in the length and number of cell tubes formed by endothelial cells co-cultured with THP-1 cells in the presence of placental factors from women with preeclampsia and uncomplicated pregnancy were found. These findings can reflect the peculiarities of the influence of macrophages on the formation of blood vessels by endothelial cells in the placenta. |
| Databáze: | OpenAIRE |
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