Efficient separation of anti‐inflammatory isolates from Polygonti rhizome by three different modes of high‐speed counter‐current chromatography

Autor: Jinqian Yu, Wenxiu Chen, Lei Zhao, Tao Yue, Wencui Yang, Xiao Wang
Rok vydání: 2022
Předmět:
Zdroj: Journal of Separation Science. 45:4012-4022
ISSN: 1615-9314
1615-9306
DOI: 10.1002/jssc.202200545
Popis: Successful isolation of 15 compounds from Polygonti rhizome was obtained by an efficient technique combined with macroporous resin column chromatography pretreatment and three different modes of high-speed counter-current chromatography for the first time. For the pretreatment, AB-8 resin was applied to remove the polysaccharides and enrich four different parts (samples I, II, III, and IV) by polarities. For the separation, sample I was separated by pH-zone-refining counter-current chromatography and seven cycle recycling mode high-speed counter-current chromatography, yielding four alkaloids (1--4); samples II-IV were further separated by the conventional high-speed counter-current chromatography, yielding seven flavonoids (5-10, 12), one steroid saponin (11), and three terpenoids (13-15). Finally, the isolates were assayed for their anti-inflammatory activities against nitric oxide production with compounds 5, 9-10, 13 showing significant anti-inflammatory activities, IC
Databáze: OpenAIRE
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