DNA barcoding, species-specific PCR and real-time PCR techniques for the identification of six Tribolium pests of stored products
| Autor: | Yi-Jiao Wang, Fu-Jun Li, Zuzana Kučerová, Wei Guo, Zhihong Li, Tao Zhang, Václav Stejskal, George P. Opit, Yi Wu, Yang Cao, Dan Luo |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitochondrial DNA animal structures Zoology Biology Real-Time Polymerase Chain Reaction 01 natural sciences DNA barcoding Article law.invention Electron Transport Complex IV 03 medical and health sciences Species Specificity Genus Phylogenetics law Botany Quarantine TaqMan Animals DNA Barcoding Taxonomic Phylogeny DNA Primers Tribolium Multidisciplinary Base Sequence fungi food and beverages DNA 010602 entomology 030104 developmental biology Identification (biology) PEST analysis Sequence Alignment |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep28494 |
| Popis: | Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored product pests in China and worldwide. They are often found or are intercepted in grain depots, flour mills, and entry-exit ports, etc. Traditionally, Tribolium species are identified according to the morphological characteristics of the adult. However, it is almost impossible to rapidly identify adult fragments and non-adult stages based on external morphological characteristics. Molecular techniques for the rapid and accurate identification of Tribolium species are required, particularly for pest monitoring and the quarantine of stored products pests. Here, we establish DNA barcoding, species-specific PCR, and real-time PCR techniques for the identification of six stored-product pest Tribolium species including T. castaneum, T. confusum, T. destructor, T. madens, T. freemani and T. brevicornis. We detected the mitochondrial DNA cytochrome oxidase subunit I (COI) barcodes for Tribolium from 18 geographic populations and 101 individuals, built a Tribolium DNA barcode library, and designed species-specific primers and TaqMan probes for the above six Tribolium species. The three techniques were applied to identify Tribolium collected from stored samples and samples captured from quarantine ports. The results demonstrated that three techniques were all able to identify the six species of Tribolium both rapidly and accurately. |
| Databáze: | OpenAIRE |
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